Skip to main content
Fig. 6 | Journal of Animal Science and Biotechnology

Fig. 6

From: miRNA sequencing analysis of healthy and atretic follicles of chickens revealed that miR-30a-5p inhibits granulosa cell death via targeting Beclin1

Fig. 6

Effect of miR-30a-5p on autophagy of granulosa cells. (A, B) The expression level of ATG5 and ATG7 after interference or overexpression of miR-30a-5p (n = 9). (C) The protein abundance of LC3 after interference or overexpression of miR-30a-5p (n = 3). (D) Adenovirus fluorescence picture of mcherry-EGFP-LC3 after interference or overexpression of miR-30a-5p. mcherry was used for marking and tracking LC3. The weakening of EGFP can indicate the fusion of lysosomes and autophagosomes to form autophagolysosomes. The yellow spots after the merge are autophagosomes (mcherry+EGFP). (E) Transmission electron micrograph of granulosa cells after interference or overexpression of miR-30a-5p. The red arrow points to autophagolysosomes. Data are presented as mean ± SEM. * P < 0.05, **P < 0.01 vs. NC

Back to article page

Journal of Animal Science and Biotechnology

ISSN: 2049-1891