Fig. 5

Beclin1 as a target gene of miR-30a-5p. (A) Comparison of miR-30a-5p seed sequences of different species. (B) Schematic diagram of the binding of miR-30a-5p and Beclin1. (C) Construction of wild-type dual-luciferase reporter gene and mutant dual-luciferase reporter gene of Beclin. (D) Luciferase assay is performed by co-transfecting wild-type or mutant-type dual-luciferase reporter gene with miR-30a-5p mimic or mimic NC in granulosa cells (n = 6). (E) The expression level of Beclin1 after interference or overexpression of miR-30a-5p (n = 9). (F) The protein abundance of Beclin1 after interference or overexpression of miR-30a-5p (n = 3). Data are presented as mean ± SEM. * P < 0.05, **P < 0.01 vs. NC