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Fig. 4 | Journal of Animal Science and Biotechnology

Fig. 4

From: miRNA sequencing analysis of healthy and atretic follicles of chickens revealed that miR-30a-5p inhibits granulosa cell death via targeting Beclin1

Fig. 4

The expression of miR-30a-5p in different chicken tissues and identification of granulosa cells. (A) qPCR was used to detect the expression of miR-30a-5p in the 13 different tissues of chicken (fat was the control group, n = 9). (B) Morphological changes of the chicken granulosa cells at 1–6 d, 100 ×. (C) Representative images of the immunofluorescence staining of the FSHR at different magnifications. FSHR: green, granulosa cell marker; DAPI: blue, nucleus; Merge: identification of granulosa cells. (D) The expression of miR-30a-5p in the granulosa cels transfected with miR-30a-5p inhibitor or miR-30a-5p mimics. Data are presented as mean ± SEM. a, b, c, d P < 0.05, **P < 0.01 vs. NC

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Journal of Animal Science and Biotechnology

ISSN: 2049-1891