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Fig. 10 | Journal of Animal Science and Biotechnology

Fig. 10

From: miRNA sequencing analysis of healthy and atretic follicles of chickens revealed that miR-30a-5p inhibits granulosa cell death via targeting Beclin1

Fig. 10

miR-30a-5p inhibits autophagy and apoptosis of granulosa cells by targeting Beclin1. (A) The expression levels of Beclin1 after the transfection of NC, Beclin1 overexpression plasmid, Beclin1 overexpression plasmid + miR-30a-5p mimics in the granulosa cells (n = 9). (B) The protein expression levels of Beclin1 after the transfection of NC, Beclin1 overexpression plasmid, Beclin1 overexpression plasmid + miR-30a-5p mimics in the granulosa cells (n = 3). (C) The protein expression levels of caspase-3 and LC3 after the transfection of NC, Beclin1 overexpression plasmid + miR-30a-5p mimics, miR-30a-5p mimics in the granulosa cells (n = 3). Data are presented as mean ± SEM. * P < 0.05, **P < 0.01 vs. NC

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Journal of Animal Science and Biotechnology

ISSN: 2049-1891