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Fig. 5 | Journal of Animal Science and Biotechnology

Fig. 5

From: Inhibiting nuclear factor erythroid 2 related factor 2-mediated autophagy in bovine mammary epithelial cells induces oxidative stress in response to exogenous fatty acids

Fig. 5

Knockdown of NFE2L2 inhibited autophagy in MAC-T cells. Scrambled non-target negative control (Si-Control) or siRNA targeting NFE2L2 (Si-NFE2L2) were used to transfect in MAC-T cells for 48 h. a Relative mRNA abundance of NFE2L2. b Western blot analysis of NFE2L2, LC3-II and p62. c Protein abundance of NFE2L2. d Protein abundance of p62. e Protein abundance of LC3-II. f MAC-T cells were co-transfected with the recombinant adenovirus mRFP-GFP-LC3 and NFE2L2 siRNA for 48 h. Representative images of autophagosomes (yellow puncta) and autolysosomes (red puncta), scale bar = 25 μm. Data were analyzed with paired t-test. The data presented are the mean ± SEM. ** P < 0.01

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Journal of Animal Science and Biotechnology

ISSN: 2049-1891