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Fig. 3 | Journal of Animal Science and Biotechnology

Fig. 3

From: Inhibiting nuclear factor erythroid 2 related factor 2-mediated autophagy in bovine mammary epithelial cells induces oxidative stress in response to exogenous fatty acids

Fig. 3

Activation of autophagy attenuated FFA-induced ROS accumulation in MAC-T cells. There were 4 experimental treatments: BSA + DMSO group, DMSO were used to treat cells for 18 h prior to use 2% BSA treating cells for an additional 24 h; 1.2 mmol/L FFA + DMSO group, DMSO were used to treat cells for 18 h before 1.2 mmol/L FFA treating cells for 24 h; BSA + 100 nmol/L Rap group, 100 nmol/L Rap were used to treat cells for 18 h followed by 2% BSA treating cells for an additional 24 h; FFA + 100 nmol/L Rap group, 100 nmol/L Rap were used to treat cells for 18 h followed by 1.2 mmol/L FFA treating cells for an additional 24 h. a Western blot analysis of LC3-II and p62. b Protein abundance of p62. c Protein abundance of LC3-II. d ROS content. Comparisons among groups were calculated using a one-way ANOVA with subsequent Bonferroni correction. The data presented are the mean ± SEM. Different superscript lowercase letters in bar charts represent significant difference (P < 0.05)

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