Fig. 2

FFA inhibited autophagy in MAC-T cells. MAC-T cells were treated with 0, 0.6, 1.2 or 2.4 mmol/L FFA for 24 h. a Western blot analysis of p62 and LC3-II. b Protein abundance of p62. c Protein abundance of LC3-II. d MAC-T cells were transfected with the recombinant adenovirus mRFP-GFP-LC3 for 48 h, and/or treated with 1.2 mmol/L FFA for another 24 h after serum-starvation for 12 h. Representative images of autophagosomes (yellow puncta) and autolysosomes (red puncta), scale bar = 25 μm. Comparisons among groups were calculated using a one-way ANOVA with subsequent Bonferroni correction. The data presented are the mean ± SEM. Different superscript lowercase letters in bar charts represent significant difference (P < 0.05)