Fig. 3

Validation of Tekt1 as a direct target of miR-199-5p. A Complementary sequences of miR-199-5p and the 3′-UTR region of the Tekt1. Red indicated the seed region of miR-199-5p; purple indicated the target 3′-UTR region of the Tekt1. B Validation of the interaction between miR-199-5p and the Tekt1 3′-UTR by a dual-luciferase reporter system. Luciferase reporters were linked with Tekt1 3′-UTRs containing either putative miR-199-5p-binding sites (3′-UTR-wt) or mutated miR-199-5p binding sites (3′-UTR-mutant). miR-199-5p mimic or mimic NC were cotransfected into HEK293T cells with luciferase-UTR constructs, and then luciferase activity was determined. The cells transfected with mimic NC plus 3′-UTR-wt were used to serve as the control group. Data were presented as mean ± S.E.M. (n = 6). Asterisk (*) indicated significant difference (***P < 0.001); ns indicated no significant difference