Fig. 8

Immunostaining and quantification of UCHL1⁺Ki67⁺ cells in porcine testis sections. A UCHL1 and Ki67 immunostaining of porcine testis sections at each age. Arrows and arrowheads point to UCHL1⁺Ki67⁺ and UCHL1⁺Ki67⁻ cells, respectively. Bar = 10 μm. B The numbers of UCHL1⁺Ki67⁺ cells (upper) and the ratios of UCHL1⁺Ki67⁺ to SOX9⁺ cells (lower) per cross-section of seminiferous cords/tubules in porcine testes at each age. C The percentages of UCHL1⁺ cells positive for Ki67 in porcine testes at each age. Data are presented as the mean ± SEM of three littermates, with 50 round cord/tubule cross-sections analyzed per individual. Different letters indicate significant differences between groups (P < 0.05)