Fig. 6

Immunostaining and quantification of UCHL1^+/−VASA⁺ cells in porcine testis sections. A UCHL1 and VASA immunostaining of porcine testis sections at each age. Arrows and arrowheads point to VASA^high and VASA^low cells, respectively, and the asterisk refers to UCHL1⁻VASA⁺ cells. Bar = 10 μm. B The numbers of UCHL1^+/−VASA⁺ cells (upper) and the ratios of UCHL1^+/−VASA⁺ to SOX9⁺ cells (lower) per cross-section of seminiferous cords/tubules in porcine testes at each age. Data are presented as the mean ± SEM of three littermates, with 50 round cord/tubule cross-sections analyzed per individual. Different letters indicate significant differences between groups (P < 0.05)