Fig. 5

Immunostaining and quantification of DBA⁺ or UCHL1⁺ cells in porcine testis sections. A Left: DBA immunostaining of porcine testis sections at each age. Bar = 10 μm. Right: the numbers of DBA⁺ cells (upper) and the ratios of DBA⁺ to SOX9⁺ cells (lower) per cross-section of seminiferous cords/tubules in porcine testes at each age. B Left: UCHL1 immunostaining of porcine testis sections at each age. Arrows and arrowheads point to UCHL1^high and UCHL1^low cells, respectively. Bar = 10 μm. Right: the numbers of UCHL1⁺ cells (upper) and the ratios of UCHL1⁺ to SOX9⁺ cells (lower) per cross-section of seminiferous cords/tubules in porcine testes at each age. Data are presented as the mean ± SEM of three littermates, with 50 round cord/tubule cross-sections analyzed per individual. Different letters indicate significant differences between groups (P < 0.05)