Fig. 3

Immunostaining and quantification of SOX9⁺ and PCNA⁺ cells in porcine testis sections. A SOX9 and PCNA immunostaining of porcine testis sections at each age. Arrows and arrowheads point to SOX9⁺PCNA⁺ and SOX9⁺PCNA⁻ cells, respectively. Bar = 10 μm. B The numbers of SOX9⁺ and SOX9⁺PCNA⁺ cells per cross-section of seminiferous cords/tubules in porcine testes at each age. C The percentages of SOX9⁺ cells positive for PCNA in porcine testes at each age. Data are presented as the mean ± SEM of three littermates, with 50 round cord/tubule cross-sections analyzed per individual. Different letters indicate significant differences between groups (P < 0.05)