Table 2 List of forward and reverse primers used for the bacterial quantification by QPCR
Target bacterial group | Forward primer 5′ to 3′ Reverse primer 5′ to 3’ | Amplicon length, bp | Tm, °C | References |
|---|---|---|---|---|
Salmonella enterica | F: TACTCAACATGGACGGCTCC R: TTTGCAAGAGAGAAGCGGGT | 630 | 59.3 57.3 | This study |
Total bacteria | F: GTGCCAGCMGCCGCGGTAA R: GACTACCAGGGTATCTAAT | 291 | 64.2 52.4 | [47] |
Lactobacillus spp. | F: AGCAGTAGGGAATCTTCCA R: CACCGCTACACATGGAG | 341 | 54.5 55.2 | [48] |
Bifidobacterium spp. | F: GCGTGCTTAACACATGCAAGTC R: CACCCGTTTCCAGGAGCTATT | 125 | 60.3 59.8 | [49] |
Enterobacteriaceae | F: ATGTTACAACCAAAGCGTACA R: TTACCYTGACGCTTAACTGC | 185 | 54.0 56.3 | [50] |
Butyryl-CoA:acetate CoA-transferase (B-CoA) | F: GCIGAICATTTCACITGGAAYWSITGGCAYATG R CCTGCCTTTGCAATRTCIACRAANGC | 530 | 67.0 64.0 | [51] |
Prevotella spp. | F: CACRGTAAACGATGGATGCC R: GGTCGGGTTGCAGACC | 514 | 58.3 56.9 | [52] |