Effect of micro-algae Schizochytrium sp. supplementation in plant diet on reproduction of female rainbow trout (Oncorhynchus mykiss): maternal programming impact of progeny

Cardona, Emilie; Segret, Emilien; Cachelou, Yoann; Vanderesse, Thibaut; Larroquet, Laurence; Hermann, Alexandre; Surget, Anne; Corraze, Geneviève; Cachelou, Frederic; Bobe, Julien; Skiba-Cassy, Sandrine

doi:10.1186/s40104-022-00680-9

Journal of Animal Science and Biotechnology

Table 5 Fatty acid profiles (% of total FA) of eggs from females fed C or MA diet sampled during Cycle 2 and Cycle 3 of the experimentation

From: Effect of micro-algae Schizochytrium sp. supplementation in plant diet on reproduction of female rainbow trout (Oncorhynchus mykiss): maternal programming impact of progeny

	Cycle 2		Cycle 3		Statistical results
Saturated fatty acids (SFA)	CB	MAB	CB	MAB	Diet	Cycle	Diet x Cycle
12:0	_	_	_	_	_	_	_
14:0	2.27 ± 0.14	0.49 ± 0.03	2.62 ± 0.14	0.45 ± 0.04	***	***	n.s
15:0	0.25 ± 0.01	0.13 ± 0.01	0.26 ± 0.02	0.14 ± 0.01	***	***	*
16:0	15.47 ± 0.50	14.10 ± 0.69	16.14 ± 0.77	15.04 ± 0.40	***	***	n.s
17:0	0.29 ± 0.02	0.17 ± 0.01	0.28 ± 0.03	0.18 ± 0.02	***	n.s	n.s
18:0	5.70 ± 0.38	4.71 ± 0.36	5.91 ± 0.25	5.50 ± 0.35	***	***	**
20:0	0.04 ± 0.01	0.05 ± 0.01	0.05 ± 0.00	0.05 ± 0.01	***	**	n.s
22:0	n.d	n.d	n.d	n.d	_	_	_
Total saturated FA (SFA)	24.03 ± 0.39	19.65 ± 0.99	25.26 ± 0.99	21.35 ± 0.69	***	***	n.s
Monounsatured fatty acids (MUFA)
16:1	5.56 ± 0.23	2.90 ± 0.26	6.12 ± 0.29	2.75 ± 0.32	***	n.s	***
17:1	0.00 ± 0.00	0.00 ± 0.00	0.08 ± 0.01	0.00 ± 0.00	***	***	***
18:1	19.92 ± 0.92	23.91 ± 1.30	17.48 ± 0.69	20.79 ± 0.77	***	***	n.s
20:1	1.50 ± 0.12	1.38 ± 0.25	1.64 ± 0.18	1.63 ± 0.21	*	***	n.s
22:1	0.04 ± 0.05	0.00 ± 0.00	0.03 ± 0.03	0.00 ± 0.00	***	n.s	n.s
Total monosatured FA	27.04 ± 0.95	28.18 ± 1.07	25.38 ± 1.02	25.17 ± 0.99	n.s	***	n.s
Polyunsatured fatty acid (PUFA)
16:2 n-4	0.27 ± 0.05	0.00 ± 0.00	0.35 ± 0.03	0.14 ± 0.02	***	***	n.s
16:3 n-4	0.28 ± 0.02	0.10 ± 0.02	0.30 ± 0.02	0.02 ± 0.02	***	***	***
16:4 n-1	0.12 ± 0.02	0.00 ± 0.00	0.14 ± 0.02	0.05 ± 0.02	***	***	n.s
18:2 n-4	0.39 ± 0.02	0.06 ± 0.01	0.41 ± 0.02	0.00 ± 0.00	**	n.s	***
18:3 n-4	0.51 ± 0.02	0.06 ± 0.02	0.52 ± 0.04	0.03 ± 0.03	***	*	n.s
18:4 n-1	0.65 ± 0.04	0.09 ± 0.02	0.68 ± 0.08	0.00 ± 0.00	***	*	***
PUFA n-6
18:2 n-6	5.14 ± 0.41	10.94 ± 1.27	4.09 ± 0.34	11.20 ± 0.71	***	**	***
18:2 n-4	0.39 ± 0.02	0.06 ± 0.01	0.41 ± 0.02	0.00 ± 0.00	**	n.s	***
18:3 n-6	0.16 ± 0.03	0.29 ± 0.06	0.06 ± 0.01	0.28 ± 0.06	***	n.s	***
20:2 n-6	1.04 ± 0.08	1.93 ± 0.24	0.95 ± 0.09	2.40 ± 0.32	***	**	***
20:3 n-6	0.60 ± 0.08	1.02 ± 0.07	0.55 ± 0.06	1.21 ± 0.17	***	**	***
20:4 n-6	1.80 ± 0.08	3.29 ± 0.20	1.74 ± 0.06	3.91 ± 0.22	***	***	*
22:2 n-6	0.08 ± 0.03	0.08 ± 0.04	0.15 ± 0.08	0.15 ± 0.04	***	***	*
22:4 n-6	0.10 ± 0.01	0.22 ± 0.04	0.12 ± 0.02	0.30 ± 0.05	***	***	***
22:5 n-6	0.15 ± 0.03	2.26 ± 0.22	0.15 ± 0.01	2.56 ± 0.19	***	***	***
Total PUFA n-6	11.29 ± 0.52	20.35 ± 1.05	10.23 ± 0.48	22.26 ± 0.85	***	n.s	***
PUFA n-3
16:4 n-3	0.05 ± 0.02	0.00 ± 0.00	0.04 ± 0.02	0.07 ± 0.01	***	***	n.s
18:3 n-3	1.00 ± 0.09	3.03 ± 0.39	0.61 ± 0.09	2.90 ± 0.20	***	***	*
18:4 n-3	0.39 ± 0.06	0.39 ± 0.09	0.32 ± 0.04	0.35 ± 0.08	**	*	n.s
20:3 n-3	0.21 ± 0.05	0.40 ± 0.07	0.14 ± 0.02	0.40 ± 0.06	***	*	*
20:4 n-3	0.79 ± 0.10	0.54 ± 0.05	0.72 ± 0.11	0.41 ± 0.06	***	***	n.s
20:5 n-3	10.91 ± 0.54	5.07 ± 0.32	10.60 ± 0.71	3.58 ± 0.45	***	***	***
21:5 n-3	0.40 ± 0.01	0.07 ± 0.01	0.48 ± 0.04	0.00 ± 0.00	***	n.s	***
22:5 n-3	4.33 ± 0.33	1.77 ± 0.15	4.99 ± 0.62	1.25 ± 0.13	***	n.s	***
22:6 n-3	18.95 ± 1.45	20.09 ± 1.46	19.70 ± 1.36	21.40 ± 0.94	***	***	n.s
Total PUFA n-3	35.57 ± 1.38	27.94 ± 1.42	36.64 ± 0.89	27.04 ± 0.64	***	***	***
Ratio
SFA/PUFA	0.50 ± 0.02	0.38 ± 0.03	0.53 ± 0.02	0.41 ± 0.02	***	***	n.s
n3 / n6	4.09 ± 0.31	1.57 ± 0.12	4.83 ± 0.25	1.38 ± 0.05	***	***	***
Index Insat	257.33 ± 6.52	253.43 ± 6.21	258.24 ± 3.97	253.14 ± 4.22	*	***	n.s
EPA/DHA	0.58 ± 0.06	0.25 ± 0.03	0.54 ± 0.07	0.17 ± 0.03	***	***	n.s
ARA/EPA	0.16 ± 0.01	0.65 ± 0.05	0.16 ± 0.01	1.11 ± 0.13	***	***	***
ARA/DHA	0.10 ± 0.01	0.16 ± 0.01	0.09 ± 0.01	0.18 ± 0.02	***	***	***

Values are means ± s.d. Two-way analysis of variance was carried out in order to assess effects of diet and cycle of reproduction. Replicates correspond to different individual egg batches (n = 10 per treatment). “n.s”: not significant; “*”: P < 0.05; “**”: P < 0.01; “***”: P < 0.001

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ISSN: 2049-1891

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