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Table 3 Reproductive performances of females, egg quality and fingerling biometric parameters according to diet treatment during reproductive Cycle 2 and Cycle 3

From: Effect of micro-algae Schizochytrium sp. supplementation in plant diet on reproduction of female rainbow trout (Oncorhynchus mykiss): maternal programming impact of progeny

 

Cycle 1

Cycle 2

Statistical results

Diets

CB

MAB

CB

MAB

Diet

Cycle

Diet × Cycle

Female biometric parameters and reproductive performances

Survival, %

90.32 ± 2.38

91.81 ± 0.71

94.10 ± 3.78

90.00 ± 14.14

n.s

n.s

n.s

Weight female at spawning time, g

3770.39 ± 349.83

3799.18 ± 310.41

5285.21 ± 623.48

5016.32 ± 510.36

n.s

***

**

Egg spawn weight, g

551.91 ± 123.11

542.98 ± 119.53

682.56 ± 135.11

675.02 ± 133.11

n.s

***

n.s

Absolute fecundity, eggs per female

7887.09 ± 2008.14

8086.81 ± 2046.72

9175.20 ± 2005.94

9988.04 ± 2173.13

n.s

***

n.s

Relative fecundity, eggs/kg of female

2108.84 ± 543.57

2134.54 ± 534.79

1764.04 ± 450.98

2007.70 ± 451.19

n.s

***

n.s

Egg quality

Egg diameter, mm

5.20 ± 1.11

5.15 ± 0.19

4.90 ± 0.48

4.64 ± 0.63

**

***

*

Coefficient of variation of egg diameter

3.29 ± 1.11

3.15 ± 0.88

3.07 ± 0.51

3.03 ± 0.43

n.s

n.s

n.s

White eggs, %

6.41 ± 8.58

6.66 ± 9.98

8.89 ± 16.72

2.88 ± 5.13

 

n.s

n.s

*

Embryo development

Fry survival after resorption, %

_

_

47.58 ± 33.65

70.29 ± 17.55

*

_

_

Fry weigth, mg

_

_

12.99 ± 1.48

12.53 ± 1.52

n.s

_

_

Malformation rate of fry, %

_

_

3.87 ± 5.55

4.30 ± 6.63

n.s

_

_

No resorption rate of fry, %

_

_

0.40 ± 0.82

0.41 ± 0.35

n.s

_

_

  1. Values are means ± s.d. Two-way analysis of variance was carried out in order to assess effects of diet and cycle of reproduction. Replicates to asses statistical effect on reproductive performances of females and egg quality correspond to different individual females (n = 90 females per treatment). Replicates to asses statistical effect on fingerling biometric parameters correspond to different individual egg batches (n = 10 egg batches per treatment). “n.s”: not significant; “*”: P < 0.05; “**”: P < 0.01; “***”: P < 0.001