Chronic corticosterone disrupts the circadian rhythm of CRH expression and m6A RNA methylation in the chicken hypothalamus

Table 8 Circadian rhythm parameters of m⁶A level and m⁶A related genes in hypothalamus, as determined by cosinor analyses

Index	Group	m⁶A	FTO	METTL3	METTL14	YTHDF1	YTHDF2	YTHDF3
Mesor	CON	99.31 ± 3.52	0.97 ± 0.04	1.13 ± 0.04	1.07 ± 0.05	0.92 ± 0.02	0.83 ± 0.02	0.94 ± 0.02
Mesor	CORT	104.6 ± 1.92	1.12 ± 0.03*	0.91 ± 0.09	ND	ND	0.54 ± 0.04*	0.73 ± 0.05*
Amplitude	CON	49.76 ± 5.04	0.24 ± 0.06	0.31 ± 0.05	0.25 ± 0.07	0.13 ± 0.03	0.18 ± 0.03	0.12 ± 0.03
Amplitude	CORT	20.76 ± 2.84**	0.28 ± 0.05	0.17 ± 0.07	ND	ND	0.11 ± 0.55	0.09 ± 0.06
Acrophase, h	CON	4.31 ± 0.45	19.82 ± 0.83	9.93 ± 0.67	9.58 ± 0.95	23.73 ± 0.98	0.87 ± 0.75	3.76 ± 1.10
Acrophase, h	CORT	ND	7.56 ± 0.58**	15.10 ± 3.43*	ND	ND	2.12 ± 1.90	1.31 ± 2.87

Values are means ± SEM. *P < 0.05, **P < 0.01, compared with CON group. ND represents not determined as there was no circadian rhythm

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