Fig. 5
Effect of chronic CORT exposure on the circadian rhythm parameters of m6A level and m6A related genes in chicken hypothalamus. The circadian rhythms of m6A level and m6A related genes mRNA expression in chicken pituitary. (A) M6A level (n = 4); (B) FTO gene; (C) METTL3 gene; (D) METTL14 gene; (E) YTHDF1 gene; (F) YTHDF2 gene; (G) YTHDF3 gene. The relative mRNA levels of m6A related genes are normalized to PPIA, n = 6 chickens per time point. The data markers in the graphs indicate the m6A related genes mRNA expression levels, and the results are expressed as the mean ± SEM. The curves represent the 24-h period determined by cosinor analysis. Data from CT2 are double-plotted. R2 values represent the degree of fitting. *P < 0.05, compared with control