Table 3 Primers used for absolute quantitative real-time polymerase chain reaction (PCR)
Target group | Sequence of primers, 5′ to 3′ | Size, bp |
|---|---|---|
Total bacteria | Forward: GTGSTGCAYGGYYGTCGTCA | 146 |
Reverse: ACGTCRTCCMCNCCTTCCTC | ||
Lactobacillus spp. | Forward: AGCAGTAGGGAATCTTCCA | 341 |
Reverse: CACCGCTACACATGGAG | ||
Enterococcus | Forward: CCCTTATTGTTAGTTGCCATCATT | 144 |
Reverse: ACTCGTTGTACTTCCCATTGT | ||
Escherichia coli | Forward: CATGCCGCGTGTATGAAGAA | 96 |
Reverse: CGGGTAACGTCAATGAGCAAA | ||
Bifidobacterium genus | Forward: TCGCGTCTGGTGTGAAAG | 243 |
Reverse: CCACA TCCAGCATCCAC |