Unveiling how vitrification affects the porcine blastocyst: clues from a transcriptomic study

Table 1 Primer sequences for genes used in gene expression analysis by quantitative real–time (qPCR)

Gene	Primer sequence 5′ to 3′	Product length, bp	Annealing Temp, C°	Accession No.	Ssc Gene ID
EMP1	F: TATACGGCGGTGAAGATGCC	130	63	NM_001099940	100,101,477
EMP1	R: AGGAAGAAGCGGTTGCCTTT	130	63	NM_001099940	100,101,477
ANXA8	F: ACCTCCACAGCTACTTTGCC	191	61	NM_001243599.1	100,155,930
ANXA8	R: CTTGTAGTCGCCACTGGTGT	191	61	NM_001243599.1	100,155,930
SLC10A1	F: CTTTCACCGGCTTCCTGCTA	154	60	XM_001927695.5	100,153,302
SLC10A1	R: GGTCCAATGACTTCAGGGGG	154	60	XM_001927695.5	100,153,302
PLET1	F: ACACCGTCGAGCTACAAGTC	172	60	NM_213744.1	396,570
PLET1	R: TGTGTGGTGTGGGTTGTGAT	172	60	NM_213744.1	396,570
BTG2	F: TTTCCTCTCCAGCCTCCTCA	142	61	NM_001097505.2	100,048,932
BTG2	R: GTAGCCAGAGCCCTTGGATG	142	61	NM_001097505.2	100,048,932
SERPINE1	F: AACCAGGCGGACTTCTCAAG	136	60	NM_213910.1	396,945
SERPINE1	R: TGCGGGCTGAGACGATAATG	136	60	NM_213910.1	396,945
GSTA4	F: GTCTGCCTTTCCTCACCTCC	84	61	NM_001243379.1	100,152,951
GSTA4	R: TTGCTGCCAGGTTCAAGGAA	84	61	NM_001243379.1	100,152,951
SPP1	F: GAGGGCAGCACTGACAGCCG	134	60	NM_214023.1	397,087
SPP1	R: GAAGGGCAGAGGCGAAGCCC	134	60	NM_214023.1	397,087
GAPDH	F: AACTGCTTGGCACCCCTGGC	150	60	AF017079.1	396,823
GAPDH	R: CTGGAGAGCCCCTCGGCCAT	150	60	AF017079.1	396,823
RN18S	F: GGGAGGAGGCTGACCGGGTT	84	60	NR_046261.1	100,861,538
RN18S	R: ATACATGCCGACGGGCGCTG	84	60	NR_046261.1	100,861,538

ISSN: 2049-1891