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Table 1 The sequences [forward (F) and reverse (R)], location on template, amplicon size (bp), and GenBank accession numbers for primers used for reverse transcription quantitative real-time polymerase chain reaction (RT-qPCR)

From: Dietary branched-chain amino acids modulate the dynamics of calcium absorption and reabsorption in protein-restricted pigs

Genes1

Sequence (5′ → 3′)

Location on template

Amplicon length, bp

GenBank accession no.

CYP24A1

F: GCTGGACAACAAAATCAATGAG

898–919

142

NM_214075.2

R: CTCATAGAGCACAAGGCAGATG

1018–1039

PTH1R

F: GGCGTCCATTACATCGTCTT

1390–1409

198

NM_214382.1

R: AAAGTCCAGTGCCAATGTCC

1568–1587

THRA

F: TGGAAAGCGAAAAAGAAAGAAC

205–226

202

NM_214190.1

R: TGAGTAGGTGGGATGGAGATTC

385–406

SLC34A1

F: TGGGCTTGTGTGACTGAGAG

2124–2143

198

NM_001044623.1

R: CCCAGTCAGAGTTGTGCGTA

2302–2321

SLC34A2

F: GAATCAGCCCGAAACAAGAG

835–854

128

NM_001256772.1

R: AAACCATCCGTCCAACAGAG

943–962

 

SLC34A3

F: TCGTCCTGGTCACAGTCC

2204–2221

192

XM_021081180.1

R: CGGGGTTCTCATAGCAGTG

2377–2395

SLC17A4

F: TTTTCAATTTCCACCCAACAAAT

1834–1856

76

XM_021098408.1

R: GGGTGGGCAGAGCTGTGT

1892–1906

TRPV5

F: AGGGTCGGTTTCTCTCGCTA

1437–1456

75

XM_021078896.1

R: GGCATAGGTGATGGTGATGACA

1490–1511

TRPV6

F: CACTTTAGGAGAGGCTTGCTG

2845–2865

147

XM_003134594.1

R: ATGACTTTATTGGAAGGTAGGAGGT

2967–2991

CALB1

F: ACGCTGACGGAAGTGGTTAC

77–96

84

NM_001130226.1

R: ATCCAGCCTTCTTTCGTGCC

141–160

VDR

F: AGGCTTCTTCAGACGGAGCATGAA

285–308

140

XM_021091108.1

R: ACTCCTTCATGCCGATGTCCA

404–424

PMCA1b

F: TCTAAAAGAAGCTGGTCATGGAACAC

3684–3709

363

XM_021091182.1

R: TCTTGATTCTGGCTTTTCTAACCCT

4022–4046

OCLN

F: TCCTGGGTGTGATGGTGTTC

633–652

144

NM_001163647.2

R: CGTAGAGTCCAGTCACCGCA

757–776

ZO1

F: AAGCCCTAAGTTCAATCACAATCT

4981–5004

130

XM_021098827.1

R: ATCAAACTCAGGAGGCGGC

5092–5110

β-Actin

F: CTGCGGCATCCACGAAACT

944–962

147

XM_021086047.1

R: AGGGCCGTGATCTCCTTCTG

1071–1090

  1. 1 CYP24A1, cytochrome P450 family 24 subfamily A member 1; PTH1R, parathyroid hormone 1 receptor; THRA, thyroid hormone receptor alpha; SLC34A1, solute carrier family 34 member 1; SLC34A2, solute carrier family 34 member 2; SLC34A3, solute carrier family 34 member 3; SLC17A4, solute carrier family 17 member 4; TRPV5, transient receptor potential cation channel subfamily V member 5; TRPV6, transient receptor potential cation channel subfamily V member 6; CALB1, calbindin-1; VDR, vitamin D receptor; PMCA1b, plasma membrane Ca ATPase 1b; OCLN, occludin; ZO1, zonula occludens 1