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Fig. 4 | Journal of Animal Science and Biotechnology

Fig. 4

From: Ryanodine receptor RyR1-mediated elevation of Ca2+ concentration is required for the late stage of myogenic differentiation and fusion

Fig. 4

The elevation of cytoplasmic Ca2+ concentration is required for myoblast fusion into myotubes. (A) Relative mRNA expression of Myf5, MyoD1, MyoG, and Mymk after the treatments with siRyR1 or thapsigargin (THA) after myogenic induction for 4 d (n = 3). (B-C) Immunostaining with myosin antibody after myogenic induction for 5 d treated with siRyR1 or THA (n = 4). (D) The relative myotube area in B. The data are presented as the mean ± SEM. *Represents significant difference between the two groups (P < 0.05)

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Journal of Animal Science and Biotechnology

ISSN: 2049-1891