Fig. 3
THA-increased cytoplasmic Ca2+ concentration was independent of RyR1. (A-B) The concentration of cytoplasmic Ca2+ labeled by Fluo-3 in C2C12 cells treated with thapsigargin (THA) for 48 h (n = 3). (C) Relative mRNA expression of related genes in C2C12 cells treated with THA on d 2 (Myf5 and MyoD1) and 4 (MyoG and Mymk) during myogenic differentiation (n = 3). (D-E) Immunostaining with RyR1 antibody of C2C12 cells after myogenic induction for 5 d treated with siRyR1 or THA (n = 5). The data are presented as the mean ± SEM. *Represents significant difference between the two groups (P < 0.05)