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Fig. 2 | Journal of Animal Science and Biotechnology

Fig. 2

From: Ryanodine receptor RyR1-mediated elevation of Ca2+ concentration is required for the late stage of myogenic differentiation and fusion

Fig. 2

The effects of RyR1 inhibition on myogenic differentiation of C2C12 myoblasts. (A-B) The concentration of cytoplasmic Ca2+ labeled by Fluo-3 treated with Dantrolene (DAN, 10 μmol/L, RyR1 inhibitor) for 48 h (n = 5). (C-D) The concentration of cytoplasmic Ca2+ labeled by Fluo-3 in C2C12 cells transfected with siRyR1 for 72 h (n = 5). (E-F) The proteins expression of RyR1 in C2C12 cells after myogenic induction for 5 d with siRyR1 transfection (n = 3). (G-H) Immunostaining with myosin antibody of C2C12 cells after myogenic induction for 5 d with DAN treatment or siRyR1 transfection (n = 6). (I) Relative mRNA expression of related genes in C2C12 cells treated with DAN on d 2 (Myf5 and MyoD1) and 4 (MyoG and Mymk) of myogenic differentiation (n = 3). (J) Relative mRNA expression of RyR1, Myf5, MyoD1, MyoG, and Mymk in C2C12 cells transfected with siRyR1 for 72 h (n = 3). *Represents significant difference between the two groups (P < 0.05)

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