Fig. 5

circPTPN4 represses mitochondria biogenesis and drives the transformation of slow-twitch to fast-twitch myofiber. (A to G) Relative mitochondrial DNA (mtDNA) content (A), mitochondrial membrane potential (B), reactive oxygen species (ROS) production (C), relative mRNA expression levels of glycogenolytic and glycolytic genes (D), relative enzymes activity of lactic dehydrogenase (LDH) and succinate dehydrogenase (SDH) (E), relative mRNA expression levels of several fast−/slow-twitch myofiber genes (F), and relative protein expression of MYH1A and MYH7B (G) in circPTPN4 overexpression CPMs. (H to N) Relative mtDNA content (H), mitochondrial membrane potential (I), ROS production (J), relative mRNA expression levels of glycogenolytic and glycolytic genes (K), relative enzymes activity of LDH and SDH (L), relative mRNA expression levels of several fast−/slow-twitch myofiber genes (M), and relative protein expression of MYH1A and MYH7B (N) in CPMs with circPTPN4 interference. In panels (G and N), the numbers shown below the bands were folds of band intensities relative to control. Band intensities were quantified by ImageJ and normalized to β-Tubulin. Data are expressed as a fold-change relative to the control. In all panels, results are shown as mean ± SEM, the statistical significance of differences between means was assessed using independent sample t-test. (*P < 0.05; **P < 0.01)