Fig. 4

circPTPN4 induces myogenetic differentiation. (A) Relative circPTPN4 expression during CPM differentiation. (B to F) MyHC immunostaining (B), myotube area (C), myoblast fusion index (D) and relative mRNA (E) and protein (F) expression levels of myoblast differentiation marker genes after overexpression of circPTPN4. (G to K) MyHC immunostaining (G), myotube area (H), myoblast fusion index (I) and relative mRNA (J) and protein (K) expression levels of myoblast differentiation marker genes with circPTPN4 inhibition. In panels (F and K), the numbers shown below the bands were folds of band intensities relative to control. Band intensities were quantified by ImageJ and normalized to β-Tubulin. Data are expressed as a fold-change relative to the control. Results are shown as mean ± SEM. In panels (A, C to E, and H to J), the statistical significance of differences between means was assessed using independent sample t-test. (*P < 0.05; **P < 0.01)