Fig. 3

circPTPN4 promotes myoblast proliferation. (A to E) EdU proliferation assay (A), the proliferation rate of myoblast (B), cell cycle analysis (C), CCK-8 assay (D), and relative mRNA levels of several cell cycle genes (E) with circPTPN4 overexpression in CPMs. (F to J) EdU proliferation assay (F), proliferation rate of myoblast (G), cell cycle analysis (H), CCK-8 assay (I), and relative mRNA levels of several cell cycle genes (J) after circPTPN4 interference in CPMs. Results are shown as mean ± SEM. In panels (B to E, and G to J), the statistical significance of differences between means was assessed using independent sample t-test. (*P < 0.05; **P < 0.01)