Butyrate in combination with forskolin alleviates necrotic enteritis, increases feed efficiency, and improves carcass composition of broilers

Table 1 The primer sequences used in RT-qPCR

Gene	Primer sequences (5′ → 3′)	Product size, bp	GenBank accession number
AvBD9	Forward: GCAAAGGCTATTCCACAGCAG	211	NM_001001611.2
AvBD9	Reverse: AGCATTTCAGCTTCCCACCAC	211	NM_001001611.2
AvBD10	Forward: TGGGGCACGCAGTCCACAAC	298	NM_001001609.2
AvBD10	Reverse: ATCAGCTCCTCAAGGCAGTG	298	NM_001001609.2
MUC2	Forward: TCTGGAGAGAGTTGTCCTGAC	105	JX284122.1
MUC2	Reverse: TCCTTGCAGCAGGAACAACT	105	JX284122.1
CLDN1	Forward: TTCCAACCAGGCTTTATGATG	140	NM_001013611.2
CLDN1	Reverse: TGCAGAGTCAGGTCAAACAGA	140	NM_001013611.2
TJP1	Forward: CATCAGCCAGAAGAGAACCAG	117	XM_037393868.1
TJP1	Reverse: CCAAGAACAAAAGTGGTATGC	117	XM_037393868.1
IL-1β	Forward: GACATCTTCGACATCAACCAG	215	XM_015297469.1
IL-1β	Reverse: CCGCTCATCACACACGACAT	215	XM_015297469.1
GAPDH	Forward: GCACGCCATCACTATCTTCC	356	NM_204305.1
GAPDH	Reverse: CATCCACCGTCTTCTGTGTG	356	NM_204305.1

ISSN: 2049-1891