Fig. 5
The effects of oleic acid on hormone secretion and follicular development in female mice. A The serum of female mice were collected to determine the E2, P4 and FSH levels (n = 5); B Representative image of vaginal smear staining to detect the estrus cycle, scale bar = 200 μm; C and D Statistics of various stages of the estrus cycle (n = 5); E Representative images of H&E-stained sections of an ovary, scale bars = 100 μm and 200 μm; F Statistics of the follicle numbers during various developmental periods (n = 5), *, primary follicle; ↓, secondary follicle; **, antral follicle; §, corpora lutea; ∞, atretic follicle. Note: Data are represented as the means ± SEMs. Significance was determined using Student’s t-test, *P < 0.05, **P < 0.01