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Fig. 3 | Journal of Animal Science and Biotechnology

Fig. 3

From: Oleic acid reduces steroidogenesis by changing the lipid type stored in lipid droplets of ovarian granulosa cells

Fig. 3

Oleic acid inhibits estradiol and progesterone synthesis in granulosa cells. The cells and media were harvested after 4-day oleic acid treatments. A P4 levels in media as detected by ELISA; B E2 levels in media as detected by ELISA; C RT-qPCR analysis of steroid hormone synthesis-related genes, including CYP11A1, CYP19A1and CYP19A3 and StAR; D Western blot analysis of steroid hormone synthesis-related genes E Quantification of the western blot analysis of CYP19A, CYP11A1 and StAR; Note: Data are means ± SEMs of three independent experiments; *P < 0.05, **P < 0.01

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