Fig. 3From: Oleic acid reduces steroidogenesis by changing the lipid type stored in lipid droplets of ovarian granulosa cellsOleic acid inhibits estradiol and progesterone synthesis in granulosa cells. The cells and media were harvested after 4-day oleic acid treatments. A P4 levels in media as detected by ELISA; B E2 levels in media as detected by ELISA; C RT-qPCR analysis of steroid hormone synthesis-related genes, including CYP11A1, CYP19A1and CYP19A3 and StAR; D Western blot analysis of steroid hormone synthesis-related genes E Quantification of the western blot analysis of CYP19A, CYP11A1 and StAR; Note: Data are means ± SEMs of three independent experiments; *P < 0.05, **P < 0.01Back to article page