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Fig. 2 | Journal of Animal Science and Biotechnology

Fig. 2

From: Oleic acid reduces steroidogenesis by changing the lipid type stored in lipid droplets of ovarian granulosa cells

Fig. 2

Oleic acid exposure increases triglyceride (TG) levels and reduces cholesteryl ester (CE) levels in granulosa cells. A Detection TG and CE levels after independent treatments with 300 μmol/L and 500 μmol/L oleic acid compared with 0 μmol/L (0.5% BSA); B RT-qPCR detecting the mRNA expression levels of the fatty acid translocase CD36 and TG synthase DGAT2 after oleic acid treatments; C mRNA expression of the cholesterol metabolism genes HMGCR, SCARB1 and ACAT2 after oleic acid treatments as determined by RT-qPCR; D Western blot analysis of TG and cholesterol metabolism-related genes; E Quantification of the western blot analysis of CD36 and DGAT2; F Quantification of the western blot analysis of HMGCR, SCARB1 and ACAT2. Note: Data are means ± SEMs of three independent experiments; *P < 0.05, **P < 0.01

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