Fig. 2From: Oleic acid reduces steroidogenesis by changing the lipid type stored in lipid droplets of ovarian granulosa cellsOleic acid exposure increases triglyceride (TG) levels and reduces cholesteryl ester (CE) levels in granulosa cells. A Detection TG and CE levels after independent treatments with 300 μmol/L and 500 μmol/L oleic acid compared with 0 μmol/L (0.5% BSA); B RT-qPCR detecting the mRNA expression levels of the fatty acid translocase CD36 and TG synthase DGAT2 after oleic acid treatments; C mRNA expression of the cholesterol metabolism genes HMGCR, SCARB1 and ACAT2 after oleic acid treatments as determined by RT-qPCR; D Western blot analysis of TG and cholesterol metabolism-related genes; E Quantification of the western blot analysis of CD36 and DGAT2; F Quantification of the western blot analysis of HMGCR, SCARB1 and ACAT2. Note: Data are means ± SEMs of three independent experiments; *P < 0.05, **P < 0.01Back to article page