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Table 6 The effect of CAL on in vitro ruminal microorganisms (Exp. 1)

From: Dietary Coleus amboinicus Lour. decreases ruminal methanogenesis and biohydrogenation, and improves meat quality and fatty acid composition in longissimus thoracis muscle of lambs

Variables CON CAL, % DM SEM P-value
10 15 20 Diet L
Total protozoa, 103/mL 4.89 4.68 4.68 4.91 0.003 0.40 0.91
 Holotricha, 103/mL 0.05 0.04 0.04 0.05 0.06 0.72 0.55
 Entodinomorpha, 103/mL 4.85 4.64 4.64 4.87 0.07 0.39 0.91
Total bacteria, 108/mL 4.74 4.31 4.51 4.48 0.06 0.08 0.27
Streptococcus bovis* 1.00ab 5.26a 0.08b 0.16b 0.70 0.01 0.76
Ruminococcus flavefaciens* 1.00 0.43 0.88 0.43 0.19 0.63 0.38
Ruminococcus albus* 1.00 0.10 3.16 18.5 3.08 0.13 0.09
Megasphaera elsdenii* 1.00 4.07 4.63 3.69 0.86 0.47 0.20
Prevotella spp.* 1.00b 8.54a 1.93ab 0.46b 1.06 0.02 0.84
Lactobacillus spp.* 1.00 0.05 0.33 0.11 0.05 0.12 0.22
Fibrobacter succinogenes* 1.00 0.57 0.28 0.02 0.10 0.27 0.40
Butyrivibrio proteoclasticus* 1.00b 5.42a 2.38ab 1.53b 0.54 < 0.01 0.47
Butyrivibrio fibrisolvens* 1.00b 15.0a 4.83ab 3.61ab 1.60 0.01 0.53
Anaerovibrio lipolytica* 1.00 0.23 0.81 0.34 0.13 0.12 0.15
Total methanogens, 107/mL 4.39a 3.87b 3.91b 3.32c 0.10 < 0.01 < 0.01
 Methanobacteriales, 106/mL 3.05a 2.85b 2.81bc 2.67c 0.04 < 0.01 < 0.01
 Methanomicrobiales, 106/mL 3.06a 2.89b 2.97b 2.67ab 0.04 0.01 0.13
  1. CON control diet, CAL Coleus amboinicus Lour. diet, SEM standard error of means, L linear response
  2. *Relative transcript abundance (∆∆ CT)
  3. Different superscripts (a, b, c) within the same row indicate significant differences (P < 0.05)