Table 6 The effect of CAL on in vitro ruminal microorganisms (Exp. 1)

Total protozoa, 10³/mL

4.89

4.68

4.68

4.91

0.003

0.40

0.91

 Holotricha, 10³/mL

0.05

0.04

0.04

0.05

0.06

0.72

0.55

 Entodinomorpha, 10³/mL

4.85

4.64

4.64

4.87

0.07

0.39

0.91

Total bacteria, 10⁸/mL

4.74

4.31

4.51

4.48

0.06

0.08

0.27

 Streptococcus bovis*

1.00^ab

5.26^a

0.08^b

0.16^b

0.70

0.01

0.76

 Ruminococcus flavefaciens*

1.00

0.43

0.88

0.43

0.19

0.63

0.38

 Ruminococcus albus*

1.00

0.10

3.16

18.5

3.08

0.13

0.09

 Megasphaera elsdenii*

1.00

4.07

4.63

3.69

0.86

0.47

0.20

 Prevotella spp.*

1.00^b

8.54^a

1.93^ab

0.46^b

1.06

0.02

0.84

 Lactobacillus spp.*

1.00

0.05

0.33

0.11

0.05

0.12

0.22

 Fibrobacter succinogenes*

1.00

0.57

0.28

0.02

0.10

0.27

0.40

 Butyrivibrio proteoclasticus*

1.00^b

5.42^a

2.38^ab

1.53^b

0.54

< 0.01

0.47

 Butyrivibrio fibrisolvens*

1.00^b

15.0^a

4.83^ab

3.61^ab

1.60

0.01

0.53

 Anaerovibrio lipolytica*

1.00

0.23

0.81

0.34

0.13

0.12

0.15

Total methanogens, 10⁷/mL

4.39^a

3.87^b

3.91^b

3.32^c

0.10

< 0.01

< 0.01

 Methanobacteriales, 10⁶/mL

3.05^a

2.85^b

2.81^bc

2.67^c

0.04

< 0.01

< 0.01

 Methanomicrobiales, 10⁶/mL

3.06^a

2.89^b

2.97^b

2.67^ab

0.04

0.01

0.13