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Table 3 The sequences of primers specific to the analyzed genes expression in the longissimus thoracis muscle of lambs

From: Dietary Coleus amboinicus Lour. decreases ruminal methanogenesis and biohydrogenation, and improves meat quality and fatty acid composition in longissimus thoracis muscle of lambs

Gene name

Primer sequence (5′ to 3′)

Reference

Forward

Reverse

SCD

GAGTACCGCTGGCACATCAA

CTAAGACGGCAGCCTTGGAT

[25]

ELOVL5

TGCTTCAGTTTGTGCTGACC

TGGTCCTTCTGGTGCTCTCT

[25]

FASN

GGAGGACGCTTTCCGTTACA

TGCTCTTCCTCACGTACCTGAA

[25]

FADS1

CTGCTGTACCTGCTGCACAT

ACGGACAGGTGTCCAAAGTC

[25]

LPL

TCATCGTGGTGGACTGGCT

CATCCGCCATCCAGTTCATA

[25]

  1. Analyzed expression of five genes: SCD stearoyl-CoA desaturase, ELOVL5 fatty acid elongase 5, FADS1 fatty acid desaturase 1, FASN fatty acid synthase and LPL lipoprotein lipase
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Journal of Animal Science and Biotechnology

ISSN: 2049-1891