Dietary Coleus amboinicus Lour. decreases ruminal methanogenesis and biohydrogenation, and improves meat quality and fatty acid composition in longissimus thoracis muscle of lambs

Table 2 The sequences of primers specific to the analyzed bacteria species

Species	Primer sequences (5′ to 3′)	Reference
Streptococcus bovis	F: TTCCTAGAGATAGGAAGTTTCTTCGG	[17]
Streptococcus bovis	R: ATGATGGCAACTAACAATAGGGGT	[17]
Ruminococcus flavefaciens	F:CGAACGGAGATAATTTGAGTTTACTTAGG	[18]
Ruminococcus flavefaciens	R: CGGTCTCTGTATGTTATGAGGTATTACC	[18]
Ruminococcus albus	F: CCCTAAAAGCAGTCTTAGTTCG	[19]
Ruminococcus albus	R: CCTCCTTGCGGTTAGAACA	[19]
Megasphaera elsdenii	F: AGATGGGGACAACAGCTGGA	[17]
Megasphaera elsdenii	R: CGAAAGCTCCGAAGAGCCT	[17]
Prevotella spp.	F: GAAGGTCCCCCACATTG	[17]
Prevotella spp.	R:CAATCGGAGTTCTTCGTG	[17]
Lactobacillus spp.	F: TATGGTAATTGTGTGNCAGCMGCCGCGGTAA	[20]
Lactobacillus spp.	R: AGTCAGTCAGCCGGACTACHVGGGTWTCTAAT	[20]
Fibrobacter succinogenes	F: GTTCGGAATTACTGGGCGTAAA	[21]
Fibrobacter succinogenes	R: CGCCTGCCCCTGAACTATC	[21]
Butyrivibrio proteoclasticus	F: TCCTAGTGTAGCGGTGAAATG	[22]
Butyrivibrio proteoclasticus	R: TTAGCGACGGCACTGAATGCCTA	[22]
Butyrivibrio fibrisolvens	F: ACACACCGCCCGTCACA	[23]
Butyrivibrio fibrisolvens	R: TCCTTACGGTTGGGTCACAGA	[23]
Anaerovibrio lipolytica	F: GAAATGGATTCTAGTGGCAAACG	[24]
Anaerovibrio lipolytica	R:ACATCGGTCATGCGACCAA	[24]

ISSN: 2049-1891