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Table.7 Effect of dietary calcium (Ca) level on ALP, OPG, OC and BMP-2 mRNA and protein expression levels in the tibia of broilers on d 211

From: Dietary calcium requirements of broilers fed a conventional corn-soybean meal diet from 1 to 21 days of age

Analyzed dietary Ca level, % ALP mRNA, RQ2 OPG mRNA, RQ2 OC mRNA, RQ2 BMP-2 mRNA, RQ2 ALP protein, RQ3 OPG protein, RQ3 OC protein, RQ3 BMP-2 protein, RQ3
0.59 1.11 0.98 1.69a 0.91ab 0.89c 1.14 1.75 1.03
0.70 1.06 1.19 1.24ab 1.07a 1.03abc 1.03 1.62 0.81
0.80 0.84 0.97 0.85bc 0.72bc 1.17ab 0.98 1.67 0.79
0.88 0.89 0.85 1.00bc 0.88abc 1.19a 0.91 1.65 0.73
0.98 1.00 1.00 1.00bc 1.00ab 1.06abc 0.88 1.88 0.79
1.09 0.87 0.74 0.65c 0.79abc 0.95bc 1.03 1.58 0.85
1.18 0.75 0.79 0.66c 0.66c 1.03abc 1.01 1.43 0.89
SEM 0.107 0.113 0.147 0.092 0.069 0.089 0.134 0.081
P-value
 Ca level 0.37 0.31 0.002 0.02 0.04 0.49 0.37 0.25
  Linear < 0.0001 0.02 0.75
 Quadratic 0.11 0.24 0.006
  1. 1Data represented the means of 6 replicates (n = 6)
  2. 2The mRNA levels were calculated as the relative quantity (RQ) of the target gene mRNA to the geometric mean of β-actin and glyceraldehyde-3-phosphate dehydrogenase mRNA, RQ = 2-ΔΔCt (Ct, threshold cycle)
  3. 3The protein levels were calculated as the relative quantity (RQ) of the target gene protein to the glyceraldehyde-3-phosphate dehydrogenase protein
  4. 4Abbreviations: ALP alkaline phosphatase, BMP-2, morphogenetic protein-2, OC osteocalcin, OPG osteoprotegerin
  5. a-cMeans within a column with unlike superscript letters were significantly different (P < 0.04)