|
Analyzed dietary Ca level, %
|
ALP mRNA, RQ2
|
OPG mRNA, RQ2
|
OC mRNA, RQ2
|
BMP-2 mRNA, RQ2
|
ALP protein, RQ3
|
OPG protein, RQ3
|
OC protein, RQ3
|
BMP-2 protein, RQ3
|
|---|
|
0.59
|
1.11
|
0.98
|
1.69a
|
0.91ab
|
0.89c
|
1.14
|
1.75
|
1.03
|
|
0.70
|
1.06
|
1.19
|
1.24ab
|
1.07a
|
1.03abc
|
1.03
|
1.62
|
0.81
|
|
0.80
|
0.84
|
0.97
|
0.85bc
|
0.72bc
|
1.17ab
|
0.98
|
1.67
|
0.79
|
|
0.88
|
0.89
|
0.85
|
1.00bc
|
0.88abc
|
1.19a
|
0.91
|
1.65
|
0.73
|
|
0.98
|
1.00
|
1.00
|
1.00bc
|
1.00ab
|
1.06abc
|
0.88
|
1.88
|
0.79
|
|
1.09
|
0.87
|
0.74
|
0.65c
|
0.79abc
|
0.95bc
|
1.03
|
1.58
|
0.85
|
|
1.18
|
0.75
|
0.79
|
0.66c
|
0.66c
|
1.03abc
|
1.01
|
1.43
|
0.89
|
|
SEM
|
0.107
|
0.113
|
0.147
|
0.092
|
0.069
|
0.089
|
0.134
|
0.081
|
|
P-value
|
|
Ca level
|
0.37
|
0.31
|
0.002
|
0.02
|
0.04
|
0.49
|
0.37
|
0.25
|
|
Linear
|
–
|
–
|
< 0.0001
|
0.02
|
0.75
|
–
|
–
|
–
|
|
Quadratic
|
–
|
–
|
0.11
|
0.24
|
0.006
|
–
|
–
|
–
|
- 1Data represented the means of 6 replicates (n = 6)
- 2The mRNA levels were calculated as the relative quantity (RQ) of the target gene mRNA to the geometric mean of β-actin and glyceraldehyde-3-phosphate dehydrogenase mRNA, RQ = 2-ΔΔCt (Ct, threshold cycle)
- 3The protein levels were calculated as the relative quantity (RQ) of the target gene protein to the glyceraldehyde-3-phosphate dehydrogenase protein
- 4Abbreviations: ALP alkaline phosphatase, BMP-2, morphogenetic protein-2, OC osteocalcin, OPG osteoprotegerin
- a-cMeans within a column with unlike superscript letters were significantly different (P < 0.04)
