Table.2 Primer sequences for real-time PCR amplification
| Gene | GenBank ID | Primer sequences | Length, pb |
|---|---|---|---|
| β-actin | NM_205518.1 | F: 5′-ACCTGAGCGCAAGTACTCTGTCT-3′ | 152 |
| R: 5′-CATCGTACTCCTGCTTGCTGAT-3′ | |||
| GAPDH | K01458 | F: 5′-CTTTGGCATTGTGGAGGGTC-3′ | 128 |
| R: 5′-ACGCTGGGATGATGTTCTGG-3′ | |||
| ALP | NM_205360.1 | F: 5′-GGAGAAGGACCCCGAATACTG-3′ | 300 |
| R: 5′-TTGACGCCGCAGAGGTAAG-3′ | |||
| OPG | XM_015283019.2 | F: 5′-ATCTCAGTCAAGTGGAGCATC-3′ | 186 |
| R: 5′-GTTCCAGTCTTCAGCGTAGTA-3′ | |||
| OC | NM_205387.3 | F: 5′-TGCTCGCAGTGCTAAAGCCTTCAT-3′ | 143 |
| R: 5′-TCAGCTCACACACCTCTCGTT-3′ | |||
| BMP-2 | NM_204358.1 | F: 5′-CCAACACCGTGTGCAGCTT-3′ | 136 |
| R: 5′-TGGAGTTCAGCTGAGGTGACAGA-3′ |