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Table.2 Primer sequences for real-time PCR amplification

From: Dietary calcium requirements of broilers fed a conventional corn-soybean meal diet from 1 to 21 days of age

Gene GenBank ID Primer sequences Length, pb
β-actin NM_205518.1 F: 5′-ACCTGAGCGCAAGTACTCTGTCT-3′ 152
   R: 5′-CATCGTACTCCTGCTTGCTGAT-3′  
GAPDH K01458 F: 5′-CTTTGGCATTGTGGAGGGTC-3′ 128
   R: 5′-ACGCTGGGATGATGTTCTGG-3′  
ALP NM_205360.1 F: 5′-GGAGAAGGACCCCGAATACTG-3′ 300
   R: 5′-TTGACGCCGCAGAGGTAAG-3′  
OPG XM_015283019.2 F: 5′-ATCTCAGTCAAGTGGAGCATC-3′ 186
   R: 5′-GTTCCAGTCTTCAGCGTAGTA-3′  
OC NM_205387.3 F: 5′-TGCTCGCAGTGCTAAAGCCTTCAT-3′ 143
   R: 5′-TCAGCTCACACACCTCTCGTT-3′  
BMP-2 NM_204358.1 F: 5′-CCAACACCGTGTGCAGCTT-3′ 136
   R: 5′-TGGAGTTCAGCTGAGGTGACAGA-3′  
  1. a Abbreviations: GAPDH glyceraldehyde-3-phosphate dehydrogenase, ALP alkaline phosphatase, OPG osteoprotegerin, OC osteocalcin, BMP-2 bone morphogenetic protein-2