Skip to main content

Table.2 Primer sequences for real-time PCR amplification

From: Dietary calcium requirements of broilers fed a conventional corn-soybean meal diet from 1 to 21 days of age

Gene

GenBank ID

Primer sequences

Length, pb

β-actin

NM_205518.1

F: 5′-ACCTGAGCGCAAGTACTCTGTCT-3′

152

  

R: 5′-CATCGTACTCCTGCTTGCTGAT-3′

 

GAPDH

K01458

F: 5′-CTTTGGCATTGTGGAGGGTC-3′

128

  

R: 5′-ACGCTGGGATGATGTTCTGG-3′

 

ALP

NM_205360.1

F: 5′-GGAGAAGGACCCCGAATACTG-3′

300

  

R: 5′-TTGACGCCGCAGAGGTAAG-3′

 

OPG

XM_015283019.2

F: 5′-ATCTCAGTCAAGTGGAGCATC-3′

186

  

R: 5′-GTTCCAGTCTTCAGCGTAGTA-3′

 

OC

NM_205387.3

F: 5′-TGCTCGCAGTGCTAAAGCCTTCAT-3′

143

  

R: 5′-TCAGCTCACACACCTCTCGTT-3′

 

BMP-2

NM_204358.1

F: 5′-CCAACACCGTGTGCAGCTT-3′

136

  

R: 5′-TGGAGTTCAGCTGAGGTGACAGA-3′

 
  1. a Abbreviations: GAPDH glyceraldehyde-3-phosphate dehydrogenase, ALP alkaline phosphatase, OPG osteoprotegerin, OC osteocalcin, BMP-2 bone morphogenetic protein-2