Table 2 Sequences of primers
Gene | Accession No. | Primer sequence (5′to 3′) | Product size, bp | Annealing temperature, °C |
|---|---|---|---|---|
β-Actin | XM_003124280.4 | F: CACGCCATCCTGCGTCTGGA R: AGCACCGTGTTGGCGTAGAG | 380 | 60 |
TLR-2 | NM_213761.1 | F: TCACTTGTCTAACTTATCATCCTCTTG R: TCAGCGAAGGTGTCATTATTGC | 162 | 60 |
TLR-4 | NM_001113039.2 | F: GCCATCGCTGCTAACATCATC R: CTCATACTCAAAGATACACCATCGG | 108 | 60 |
TNF-α | NM_214022.1 | F: CGTCGCCCACGTTGTAGCCAAT R: GCCCATCTGTCGGCACCACC | 128 | 62 |
IL-6 | NM_001252429.1 | F: TGGCATCTGCCTTCCCTACC R: CAGAGATTTTGCCGAGGATG | 132 | 60 |
IL-8 | NM_213867.1 | F: TTCGATGCCAGTGCATAAATA R: CTGTACAACCTTCTGCACCCA | 176 | 60 |
| Â | NM-001204296.1 | F: ACACCCATGGGCGCTATGT R: GCCTGCAGAAACCTGCTCAT | 68 | 55.7 |
| Â | XM-007465997.1 | F: CTGCTCCGGGTCCTGTGGGA R: CCCGCTGGCTGGTGCGATAC | 100 | 59 |
PG1–5 | XM_005669497.2 | F: GTAGGTTCTGCGTCTGTGTCG R: CAAATCCTTCACCGTCTACCA | 166 | 60 |
pBD-2 | AY506573.1 | F: CCAGAGGTCCGACCACTACA R: GGTCCCTTCAATCCTGTTGAA | 88 | 59 |