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Fig. 4 | Journal of Animal Science and Biotechnology

Fig. 4

From: Alterations in intestinal microbiota composition coincide with impaired intestinal morphology and dysfunctional ileal immune response in growing-finishing pigs under constant chronic heat stress

Fig. 4

Constant chronic heat stress provokes the immune response in the ileum by NF-κB pathway. Relative mRNA expression levels of TLR-2 (A), TLR-4 (A), TNF-α(A), IL-6 (A), IL-8 (A), mucin-1(B), mucin-2(B), PG1–5(B), pBD2(B) in the ileum of growing-finishing pigs were detected by Real Time-PCR. Protein expression of P65 (C) in the ileum of growing-finish pigs was detected by western blotting and the bar graphs showed the protein band intensity. All data were expressed as the mean ± SEM (n = 8 for TN and PF, n = 7 for HS). TN, thermal neutral conditions (25 ± 1 °C); HS, heat stress conditions (35 ± 1 °C); PF, pair-fed with HS under TN conditions (25 ± 1 °C). Differences were determined by one-way ANOVA followed by LSD test. Groups without a common letter mean significant differences (P < 0.05). Abbreviations: PG1–5, protegrin 1–5; pBD2, porcine beta-defensin 2; TLR-2, toll-like receptor 2; TLR-4, toll-like receptor 4; TNF-α, tumor necrosis factor-α; IL-6, interleukin- 6; IL-8, interleukin- 8

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