Fig. 3

Effects of constant chronic heat stress on the expression of heat shock protein and tight junction proteins of small intestine sections in growing-finishing pigs. A, B, C presented duodenum, jejunum, and ileum respectively. TN, thermal neutral conditions (25 ± 1 °C); HS, heat stress conditions (35 ± 1 °C); PF, pair-fed with HS under TN conditions (25 ± 1 °C). The bar graphs showed the protein band intensity. All data were expressed as the mean ± SEM (n = 8 for TN and PF, n = 7 for HS). Differences were determined by one-way ANOVA followed by LSD test. Groups without a common letter mean significant differences (P < 0.05). Abbreviations: HSP 70, heat shock protein 70, ZO-1, zonula occluden-1