Table 3 Antioxidant related and protein degradation related genes’ primers used for quantitative polymerase chain reaction analysis
Gene name | Accession No. | Primer (5' to 3') |
|---|---|---|
mTOR | XM_417614.2 | F: GGTGATGACCTTGCCAAACT |
R: CTCTTGTCATCGCAACCTCA | ||
Antioxidant related | ||
 NRF2 | NM_205117.1 | F: GAGAAAGCCTTGCTGGCTCA |
R: TGAAGTATCTGTGCTCTGCGAA | ||
 CAT | NM_001030762.3 | F: GCGCCCCGAACTATTATCCA |
R: ATACGTGCGCCATAGTCAGG | ||
 SOD1 | NM_205064.1 | F: GGCAATGTGACTGCAAAGGG |
R: ATGCAGTGTGGTCCGGTAAG | ||
 SOD2 | NM_204211.1 | F: TACAGCTCAGGTGTCGCTTC |
F: GCGAAGGAACCAAAGTCACG | ||
 GSH-PX1 | NM_001277853.2 | F: TGCGCCCGATGTTTTCAAAG |
R: AACGTTACCCAGACTCACGG | ||
 GSH-PX4 | NM_001346448.1 | F: GGGTGAAGTTCGACATGTTCAG |
R: GTTCCACTTGATGGCATTCCC | ||
Protein degradation related genes | ||
 μ-Calpain | NM_205303 | F: CACACAAGGAGGCCGACTTC |
R: TCCGCTGTGTCTGACTGCTT | ||
 20S proteasome C1 subunit | AB_001935 | F: TGAGGAACAAGGAGCCCATCT |
R: TGCCCTTGTACTGATACACCATGT | ||
 m-Calpain large subunit | D_38026 | F: GTGGCTCGGTTTGCTGATG |
R: AATCAAGCACCGGACACAATT | ||
 Caspase 3 | AF_083029 | F: GGAACACGCCAGGAAACTTG |
R: TCTGCCACTCTGCGATTTACA | ||
 Cathepsin B | U_18083 | F: GCTACTCGCCTTCCTACAAGGA |
R: GCGAGGGACACCGTAGGAT | ||
 Atrogin1/MAFbx | XM_418451 | F: CCAACAACCCAGAGACCTGT |
R: GGAGCTTCACACGAACATGA | ||