Table 2 Amino acid transporter and lipid metabolism-related genes’ primers used for a quantitative polymerase chain reaction
Gene name | Accession No. | Primer (5' to 3') |
|---|---|---|
Amino acid transporter | ||
 SLC7A1/ CAT1 | EU360441.1 | F: CGAACAACAGAGGAGACAGATAA |
R: GGGACACAGTATGGCTTTGA | ||
 SLC43A2/ LAT4 | XM 415803.4 | F: GACTCGCAGCATCCCTAAAT |
R: GTGTCAGAGAAGTGGACGATATG | ||
 SLC7A5/ LAT1 | NM_001030579.1 | F: GATTGCAACGGGTGATGTGA |
R: CCCCACACCCACTTTTGTTT | ||
 SLC1A4/ ASCT1 | XM_001232899.2 | F: TTGGCCGGGAAGGAGAAG |
R: AGACCATAGTTGCCTCATTGAATG | ||
 SLC1A1/ EAAT3 | XM_424930 | F: ACCCTTTTGCCTTGGAAACT |
R: TTGAGATGTTT GCGTGAAG | ||
 SLC6A19/ B0AT | XM_419056 | F: TGCGTAGGGTTTTGTGTTGG |
R: AACTCCAGACT CCCACACTG | ||
Lipid metabolism | ||
 ACC1 | NM_205505 | F: AATGGCAGCTTTGGAGGTGT |
R: TCTGTTTGGGTGGGAGGTG | ||
 FADS1 | NC_006092.4 | F: TCACTTGTGGAGGTAAGCATC |
R: GGCGAGAAAGGAGAGGAGTC | ||
 SREBP1c | AY029224 | F: GCCCTCTGTGCCTTTGTCTTC |
R: ACTCAGCCATGATGCTTCTTCC | ||
 PPARα | AF163809 | F: AGACACCCTTTCACCAGCATCC |
R: AACCCTTACAACCTTCACAAGCA | ||
 SCD1 | NM_205064.1 | F: TTGTCTGATGGAGATCATGGCTTC |
R: TGCTTGCCTTCAGGATTAAAGTGAG | ||
 CPT-1 | AY675193 | F: GGGACCTGAAACCAGAGAACG |
R: ACAGAGGAGGGCATAGAGGATG | ||
 ACOX1 | NM_001012578 | F: GCCAGGTGGACTTGGAAAGA |
R: GCTGCCGTATAGGAACAATGAAG | ||
 FABP1 | NM_204192.3 | F: AGAAGGCCAAGTGTATTGTTAACAT |
R: GTGATGGTGTCTCCGTTGAGTTC | ||
 β-actin | NM 205518.1 | F: ACCGGACTGTTACCAACACC |
R: CCTGAGTCAAGCGCCAAAAG | ||