Table 2 List of gene primer sequences a
From: Soya saponin improves egg-laying performance and immune function of laying hens
Gene name | Prime sequence(5' to 3') | Product size, bp | |
|---|---|---|---|
GnRH1 | F | GGCTCAACACTGGTCTTATGG | 202 |
R | TCTTCTGGCTTCTCCTTCG | ||
ERR | F | GTACGGCTCTACTACACTCAGTTATGC | 160 |
R | CTGCTGGCTGTGGTGATGGATG | ||
PGR | F | GTGTCGCTTGAGGAAGTGCTGTC | 116 |
R | CGGCTGGCTGCTGAAGTGC | ||
LHR | F | CGTCCTCATAACCAGCCACTACAAG | 119 |
R | TCTGAGCATCCACCGAAGCAATG | ||
FSHR | F | GTCTCACCTGCTTGCTGATTCTCC | 99 |
R | CCTTGATCTCCTGGCAGATGAATATCC | ||
PPAR-γ | F | TCCTTCCCGCTGACCAAA | 212 |
R | TCCTGCACTGCCTCCACA | ||
TNF-α | F | GAGCGTTGACTTGGCTGTC | 64 |
R | AAGCAACAACCAGCTATGCAC | ||
IL-1β | F | ACTGGGCATCAAGGGCTA | 131 |
R | GGTAGAAGATGAAGCGGGTC | ||
IFN-γ | F | AGCTGACGGTGGACCTATTATT | 259 |
R | GGCTTTGCGCTGGATTC | ||
IL-4 | F | AGACAAATAACAAAACTGAGC | 212 |
R | TTGGTGGAAGAAGGTACG | ||
NF-κB | F | GTGTGAAGAAACGGGAACTG | 203 |
R | GGCACGGTTGTCATAGATGG | ||
Caspase1 | F | CGGCCAGCGCCATCTTCATT | 347 |
R | AGGGAGCTGTCACAGTGCGT | ||
IL-12 | F | AAGGTGCAGAAGCAGAGGAC | 88 |
R | TTGTGTTGCTCTGACTGTTGG | ||
TGF-β | F | TCATCACCAGGACAGCGTTA | 109 |
R | TGTGATGGAGCCATTCATGT | ||
β-actin | F | GAGAAATTGTGCGTGACATCA | 152 |
R | CCTGAACCTCTCATTGCCA | ||