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Fig. 3 | Journal of Animal Science and Biotechnology

Fig. 3

From: Soya saponin improves egg-laying performance and immune function of laying hens

Fig. 3

The effects of SS on peripheral blood lymphocyte ratio, stimulation index, and the antibody titer of BSA. Peripheral blood lymphocyte stimulation index and lymphocyte ratio at the end of 5th week were showed in A and B, (n = 6). C and D showed the peripheral blood lymphocyte stimulation index and lymphocyte ratio at the end of 10th week, (n = 8). The results of BSA antibody titer were showed in E, (n = 8). Among them, ** represents an extremely significant difference (P < 0.01), * was judged as a significant difference (0.01 < P < 0.05), the same below. Our analysis steps for flow cytometry results were as follows. At first, we use the CD45 ring gate to eliminate the interference of red blood cells. In the gate of CD45+, T lymphocytes were labeled with CD3+ and their ratios were obtained. B lymphocytes and monocytes were labeled with Bu1+ and Mon+, and their ratios were obtained. The detailed flow analysis density map could be tracked in supplementary Figs. 1 and 2

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