Table 2 Nucleotide sequences of primers
Target genes | Primer sequences (5′to 3′) | Used for |
|---|---|---|
SREBP1 | F: CTACCGCTCATCCATCAACG | Real-time PCR |
R: CTGCTTCAGCTTCTGGTTGC | ||
FASN | F: CGTCATCACCGTCTATC | Real-time PCR |
R: GTAGGCTCCTCCCATC | ||
SCD | F: CTATGCGGGGCTACTT | Real-time PCR |
R: GGATGGCTGGAATGAA | ||
GR | F: CTTCCATCCGCCCTTCA | Real-time PCR |
R: TCGCATCTGTTTCACC | ||
METTL3 | F: GCTCCATCCAGGCCCATAAG | Real-time PCR |
R: CCCACTCACCGTATCGATGG | ||
METTL14 | F: GTGATTCTCCTGGAGCCACC | Real-time PCR |
R: TGGGGTCCAGAGTCTTCGTT | ||
FTO | F: TGAAGGTAGCGTGGGACATAGA | Real-time PCR |
R: TGAAGGTAGCGTGGGACATAGA | ||
YTHDF1 | F: ACAAGCGTTGACCCTCAGAGA | Real-time PCR |
R: TGTTCCCCAAGCTGAGAAGG | ||
YTHDF2 | F: TCCTACTCTCTGGGTGAGGC | Real-time PCR |
R: GCGTAATTGCTGCTGTAGCC | ||
YTHDF3 | F: CCACCAACACTGGTGCAAAG | Real-time PCR |
R: GCCCACACCCCTATTACGAG | ||
FTO Fragment | F: AAAACTGAGGGGGGAT | ChIP PCR |
R: ACAACTGTGGGCAAGG | ||
YTHDF2 Fragment 1 | F: GTGCTTGTTGCTACTCGT | ChIP PCR |
R: CCATAGAGGAACCCAATC | ||
YTHDF2 Fragment 2 | F: GGGCTCAGGTGGTTTGTT | ChIP PCR |
R: CACGTCCAGCGATTCATC | ||
YTHDF1 Fragment | F: GCAGGTATTTTGACACTT | ChIP PCR |
R: TATGCATTGACCAGAACT |