GR-mediated transcriptional regulation of m6A metabolic genes contributes to diet-induced fatty liver in hens

Table 1 Ingredient and calculated composition of the diets (based on air-dried weight)

Items	CON	HELP
Ingredient, %
Corn	62.1	68.21
Soybean meal	26	13
Soybean oil	0	6.5
Limestone	8.9	8.9
Lysine	0	0.34
Methionine	0	0.14
Premix^a	3	3
Total	100	100
Calculated composition, %
Metabolizable energy, kcal/kg	2,610	3,100
Crude protein	16.9	12.1
Calcium	3.21	3.17
Available P	0.59	0.58
Lysine	0.80	0.77
Methionine	0.38	0.34

^aThe premix was composed of the following per kg diet: VA 9,000 IU; VD₃, 3,600 IU; VE, 12 IU; VK₃, 3.00 mg; VB₁, 2.00 mg; VB₂, 6.90 mg; VB₆, 2.70 mg; VB₁₂, 0.02 mg; D-Biotin, 0.23 mg; nicotinic acid, 31.20 mg; Folic acid, 1.00 mg; VB₅, 10.80 mg; Choline chloride, 0.30 g; Fe (as FeSO₄), 60.00 mg; Cu (as CuSO₄), 12.00 mg; Mn, 90.00 mg; Zn (as ZnSO₄), 90.00 mg; I (as KI), 0.80 mg; Se (as Na₂SeO₃), 0.30 mg

ISSN: 2049-1891