Table 1 Effect of lovastatin on in vitro ruminal methane production
From: Lovastatin as a supplement to mitigate rumen methanogenesis: an overview
Source of Lv | Donor animals and experimental diet | In vitro gas production technique | Lv, mg/L | Methane Inhibition, % | Reference |
|---|---|---|---|---|---|
Pure cultures of methanogenic archaea | |||||
Sigma-Aldrich, St Louis, MO, USA | Technique: Hungate tubes Culture medium: BRN Inoculum: Methanobrevibacter strains | 4 | 99 | [26] | |
Fermented rice straw extracts | Technique: serum bottles Culture medium: Balch 1 (DSMZ, Germany) Inoculum: M. smitthii | 50 | 100 | [27] | |
Sigma-Aldrich, St Louis, MO, USA | Technique: Hungate tubes Culture medium: SAB Inoculum: M. smitthii | 4 | 100 | [38] | |
Ruminal-based inoculum and diets | |||||
Sigma-Aldrich, St Louis, MO, USA | Ruminant: bovine Diet: f:ca ratio of (50:50) | Technique: fermentation bottles Substrate: diet with a f:c ratio of (50:50) Inoculum: ruminal fluid | 5 | NSb | [39] |
Sigma-Aldrich, GmbH, Buchs, Switzerland | Ruminant: bovine Diet: hay, ryegrass, and concentrate | Technique: RUSITEC system Substrate: diet with a f:c ratio of (50:50) Inoculum: ruminal fluid | 150 | 40 | [40] |
Fermented rice straw | Ruminant: bovine Diet: f:c ratio of (40:60) | Technique: calibrated glass syringes Substrate: fermented rice straw Inoculum: ruminal fluid | 4.3 | 24 | [34] |
Sigma-Aldrich, St Louis, MO, USA | Ruminant: bovine Diet: f:c ratio of (60:40) | Technique: serum bottles Substrate: diet with a f:c ratio of (50:50) Inoculum: ruminal fluid | 3.2 | NS | [41] |
Fermented rice | Ruminant: sheep Diet: hay | Technique: Hungate tubes Substrate: fermented rice Inoculum: ruminal fluid | 40 | 9.6 | [36] |
Fermented purple corn stover | Ruminant: dairy steers Diet: no reported | Technique: serum bottles Substrate: fermented purple corn cob Inoculum: ruminal fluid | 29.5 | 14.6 | [42] |
Fermented Oat straw | Ruminant: bovine Diet: f:c ratio of (60:40) | Technique: serum bottles Substrate: diet with a f:c ratio of (30:70) Inoculum: ruminal fluid | 150 | 38 | [31] |
Simvastatin, Sigma-Aldrich, Prague, Czech Republic | Ruminant: bovine Diet: f:c ratio of (70:30) | Technique: serum bottles Substrate: diet with a f:c ratio of (64:36) Inoculum: ruminal fluid | 100 | 26.2 | [43] |