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Fig. 1 | Journal of Animal Science and Biotechnology

Fig. 1

From: Transcriptome sequencing analysis for the identification of stable lncRNAs associated with bovine Staphylococcus aureus mastitis

Fig. 1

Workflow of this study. Five related experiments were designed. The bovine mammary gland was challenged with different concentrations of S. aureus (in vivo). Bovine mammary gland alveolar cells (Mac-T cells) were challenged with different S. aureus strains (in vitro). Mac-T cells were subjected to folic acid treatment and S. aureus challenge, and the association between SNPs around key long non-coding RNA (lncRNA) and hematological parameters (HP) was tested at the population level. Finally, the function of lncRNA was validated by gene knockdown and overexpression. S. aureus: Staphylococcus aureus; FA: folic acid; PRANCR: progenitor renewal associated non-coding RNA; TNK2-AS1: TNK2 antisense RNA 1. iC: mammary gland challenged with saline; iL: mammary challenged with low concentration of S. aureus; iH: mammary challenged with high concentration of S. aureus. CC: cells control; CL: cells challenged with Strain L; CM: cells challenged with Strain M; CMM: cells challenged with Strain MM; FC: cells treated by FA; FL: cells treated by FA and challenged with Strain L; FM: cells treated by FA and challenged with Strain M; FMM: cells treated by FA and challenged with Strain MM

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