Fig. 1From: Transcriptome sequencing analysis for the identification of stable lncRNAs associated with bovine Staphylococcus aureus mastitisWorkflow of this study. Five related experiments were designed. The bovine mammary gland was challenged with different concentrations of S. aureus (in vivo). Bovine mammary gland alveolar cells (Mac-T cells) were challenged with different S. aureus strains (in vitro). Mac-T cells were subjected to folic acid treatment and S. aureus challenge, and the association between SNPs around key long non-coding RNA (lncRNA) and hematological parameters (HP) was tested at the population level. Finally, the function of lncRNA was validated by gene knockdown and overexpression. S. aureus: Staphylococcus aureus; FA: folic acid; PRANCR: progenitor renewal associated non-coding RNA; TNK2-AS1: TNK2 antisense RNA 1. iC: mammary gland challenged with saline; iL: mammary challenged with low concentration of S. aureus; iH: mammary challenged with high concentration of S. aureus. CC: cells control; CL: cells challenged with Strain L; CM: cells challenged with Strain M; CMM: cells challenged with Strain MM; FC: cells treated by FA; FL: cells treated by FA and challenged with Strain L; FM: cells treated by FA and challenged with Strain M; FMM: cells treated by FA and challenged with Strain MMBack to article page