Fig. 4
From: Single-cell RNA-sequencing reveals the dynamic process and novel markers in porcine spermatogenesis
Single-cell porcine spermatogonia trajectories reveal transitions with differentiation and initiation of meiosis. (A) Focused analysis (t-SNE and clustering) of the mitosis and initiation of meiotic germ cells reveals differentiation progression of spermatogonia. (B) t-SNE plot reveal the states in spermatogonia differentiation. (C) Dot plot depicts the expression of marker genes in each state. The dot size represents the percentage of cells expressed and the color intensity represents relative expression level. (D) The correlation across each state and illustrated by Pearson correlation analysis. (E) Expression patterns of new defined marker genes for undifferentiated and differentiating spermatogonia visualized in t-SNE plots. (F) Heatmap of marker genes from each state. Top, cell types; Left, number of marker genes; Right, representative GO terms and representative genes