Table 2 Receiver operating characteristic (ROC) curve analysis showing the area under the curve (AUC), standard deviation (SD), level of significance (P-value), cut-off value, sensitivity, specificity, positive predictive value (PPV), negative predictive value (NPV) and ODDs ratio of all sperm parameters at 0 h post-thaw for discriminating between subfertile (n = 13) and highly fertile (n = 12) bulls
From: Sperm chromatin condensation as an in vivo fertility biomarker in bulls: a flow cytometry approach
Sperm parameter at 0 h after thawing | AUC | SD | P-value | Cut-off | Sensitivity | Specificity | PPV | NPV | ODDs ratio |
|---|---|---|---|---|---|---|---|---|---|
Normal morphology, % | 0.54 (0.30–0.77) | 0.12 | 0.765 | 90.12% | 69.23% (42.37–87.32) | 50.00% (25.38–74.62) | 60.00% | 60.00% | 1.39 |
Viability, % | 0.64 (0.41–0.87) | 0.12 | 0.231 | 54.90% | 76.92% (49.74–91.82) | 58.33% (31.95–80.67) | 66.66% | 70.00% | 1.85 |
Total motility, % | 0.68 (0.47–0.89) | 0.11 | 0.128 | 49.87% | 69.23% (42.37–87.32) | 66.67% (39.06–86.19) | 69.23% | 66.67% | 2.08 |
Progressive motility, % | 0.73 (0.53–0.93) | 0.10 | 0.050* | 23.13% | 61.54% (35.52–82.29) | 83.33% (55.20–97.04) | 80.00% | 66.67% | 3.69 |
Viable CMA3+ sperm, % | 0.78 (0.57–0.98) | 0.10 | 0.019* | 2.72% | 76.92% (49.74–91.82) | 83.33% (55.20–97.04) | 83.33% | 76.92% | 4.62 |
Viable CMA3+ sperm, FI | 0.70 (0.49–0.91) | 0.11 | 0.092 | 907.40 | 76.92% (49.74–91.82) | 66.67% (39.06–86.19) | 71.43% | 72.73% | 2.31 |
Total CMA3+ sperm, % | 0.74 (0.51–0.96) | 0.11 | 0.044* | 19.68% | 100.00% (77.19–100.00) | 66.67% (39.06–86.19) | 76.47% | 100.00% | 3.00 |
Total CMA3+ sperm, FI | 0.76 (0.56–0.96) | 0.10 | 0.030* | 1045.70 | 100% (77.19–100.00) | 50.00% (25.38–74.62) | 68.42% | 100.00% | 2.00 |
Viable CMA3+ sperm, % + Progressive motility, % | 0.83 (0.66–1.00) | 0.09 | 0.005* | −0.42 | 69.23% (42.37–87.32) | 83.33% (55.20–97.04) | 81.82% | 71.43% | 4.15 |