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Table 2 Receiver operating characteristic (ROC) curve analysis showing the area under the curve (AUC), standard deviation (SD), level of significance (P-value), cut-off value, sensitivity, specificity, positive predictive value (PPV), negative predictive value (NPV) and ODDs ratio of all sperm parameters at 0 h post-thaw for discriminating between subfertile (n = 13) and highly fertile (n = 12) bulls

From: Sperm chromatin condensation as an in vivo fertility biomarker in bulls: a flow cytometry approach

Sperm parameter at 0 h after thawing AUC SD P-value Cut-off Sensitivity Specificity PPV NPV ODDs ratio
Normal morphology, % 0.54 (0.30–0.77) 0.12 0.765 90.12% 69.23% (42.37–87.32) 50.00% (25.38–74.62) 60.00% 60.00% 1.39
Viability, % 0.64 (0.41–0.87) 0.12 0.231 54.90% 76.92% (49.74–91.82) 58.33% (31.95–80.67) 66.66% 70.00% 1.85
Total motility, % 0.68 (0.47–0.89) 0.11 0.128 49.87% 69.23% (42.37–87.32) 66.67% (39.06–86.19) 69.23% 66.67% 2.08
Progressive motility, % 0.73 (0.53–0.93) 0.10 0.050* 23.13% 61.54% (35.52–82.29) 83.33% (55.20–97.04) 80.00% 66.67% 3.69
Viable CMA3+ sperm, % 0.78 (0.57–0.98) 0.10 0.019* 2.72% 76.92% (49.74–91.82) 83.33% (55.20–97.04) 83.33% 76.92% 4.62
Viable CMA3+ sperm, FI 0.70 (0.49–0.91) 0.11 0.092 907.40 76.92% (49.74–91.82) 66.67% (39.06–86.19) 71.43% 72.73% 2.31
Total CMA3+ sperm, % 0.74 (0.51–0.96) 0.11 0.044* 19.68% 100.00% (77.19–100.00) 66.67% (39.06–86.19) 76.47% 100.00% 3.00
Total CMA3+ sperm, FI 0.76 (0.56–0.96) 0.10 0.030* 1045.70 100% (77.19–100.00) 50.00% (25.38–74.62) 68.42% 100.00% 2.00
Viable CMA3+ sperm, % + Progressive motility, % 0.83 (0.66–1.00) 0.09 0.005* −0.42 69.23% (42.37–87.32) 83.33% (55.20–97.04) 81.82% 71.43% 4.15