Fig. 4
From: Sperm chromatin condensation as an in vivo fertility biomarker in bulls: a flow cytometry approach
Receiver operating characteristic (ROC) curves for A the percentage of morphologically normal sperm; B the percentage of viable sperm; C the percentage of total motile sperm; D the percentage of progressively motile sperm; E the percentage of viable sperm (Yo-Pro-1−) showing chromatin decondensation (chromomycin A3-positive; CMA3+); F the percentage of progressively motile sperm and the percentage of viable sperm (Yo-Pro-1−) showing chromatin decondensation (CMA3+); G the percentage of total sperm showing chromatin decondensation (CMA3+); H the CMA3 fluorescence intensity of viable, chromatin-decondensed sperm (CMA3+/Yo-Pro-1−); and I the CMA3 fluorescence intensity of total chromatin-decondensed sperm (CMA3+) for discriminating between subfertile (n = 13) and fertile (n = 12) bulls. The ROC curve of each parameter shows the area under the curve (AUC) and the corresponding significance level (p-value; P), and the cut-off point (Co) of the sensitivity (SE) and specificity (SP) values