Skip to main content
Fig. 1 | Journal of Animal Science and Biotechnology

Fig. 1

From: Sperm chromatin condensation as an in vivo fertility biomarker in bulls: a flow cytometry approach

Fig. 1

Flow cytometry histograms of a representative sperm sample and negative control without chromomycin A3 (CMA3). Sperm samples were stained with both CMA3/Yo-Pro-1, whereas CMA3 was omitted in negative controls (Yo-Pro-1 staining). Yo-Pro-1 staining was used to stablish viable (LIVE), non-viable (DEAD) and total (TOTAL; viable + non-viable sperm) populations, whereas negative control was used to stablish CMA3-positive (CMA3+) and CMA3-negative (CMA3) populations. CMA3 histograms of the sample and negative control are represented for viable, non-viable and total sperm populations. CMA3 was acquired with the Violet610 channel (610/20), whereas Yo-Pro-1 was collected with the FITC channel (525/40)

Back to article page